http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104789539-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-90 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y504-99016 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y204-01245 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1051 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-54 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-12 |
| filingDate | 2015-04-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2017-10-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2017-10-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104789539-B |
| titleOfInvention | A kind of mutant of trehalose synthase and its preparation method and application |
| abstract | The invention discloses a kind of mutant of trehalose synthase and its preparation method and application, belong to genetic engineering and enzyme engineering field.The glutamic acid mutation of the 289th near Thermobifida fusca YX trehalose synthase activated centre is obtained mutant E289G by the present invention into glycine；By the Histidine mutagenesis of the 295th into asparagine mutant H295N；The methionine of the 344th is mutated into lysine mutation body M344K；The methionine of the 367th is mutated into leucine mutant M367L, and double mutation are carried out on the basis of H295N and obtains mutant H295N/E289G, H295N/M344K, H295N/M367L, H295N/M344K/M367L.Even if mutant is realized containing certain glucose in substrate, the transformation efficiency that trehalose synthase prepares trehalose will not be still greatly affected, with higher industrial value. |
| priorityDate | 2015-04-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 51.