http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104762391-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6851 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-645 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2015-04-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2017-03-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2017-03-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104762391-B |
| titleOfInvention | A kind of method of prediction Wheat Seedling sharp eyespot resistance |
| abstract | The present invention provides a kind of method of prediction Wheat Seedling sharp eyespot resistance, comprises the following steps that：（A）12d is cultivated after the wheat grain seed soaking of wheat breed to be measured or strain is sprouted, inoculated；（B）Take wheat seedling base portion to be measured to include the 1cm stalk of sheath, weigh, be designated as A, unit is mg；Extract stalk STb gene, as DNA sample to be measured, volume is designated as B, unit is μ L；(C) with the DNA sample to be measured of step B acquisition as template, with SEQ.ID NO:1 is forward primer, SEQ.ID NO:2 is reverse primer, carries out fluorescence quantitative PCR detection, obtains CP value；Repeat to detect CP meansigma methodss C obtaining three detections twice again；(D) according to formula E=[B × 10 (31.784‑C)/3.562 ]/A calculates the DNA content E of wheat seedling base portion stalk Rhizoctonia cerealis to be detected；(E) the DNA content E of the base portion stalk Rhizoctonia cerealis of control sample Huaihe River wheat 18 seedling is obtained with above-mentioned identical A D step 1 ；(F) pass through E and E 1 The sharp eyespot resistance to predict the wheat seedling phase for the ratio. |
| priorityDate | 2015-04-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
| Predicate | Subject |
|---|---|
| isDiscussedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/compound/CID5974 http://rdf.ncbi.nlm.nih.gov/pubchem/substance/SID450766143 |
Total number of triples: 14.