http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104745626-B
Outgoing Links
Predicate | Object |
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classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01K67-027 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-85 |
filingDate | 2014-12-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2018-05-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2018-05-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-104745626-B |
titleOfInvention | A kind of fast construction method of conditional gene knockout animal model and application |
abstract | The present invention relates to a kind of fast construction method of conditional gene knockout animal model, it is completed using CRISPR/Cas9 technologies.Specific method can beļ¼The gRNA target sequences that CRISPR/Cas9 systems are directed to animal gene to be knocked out are designed, design corresponding promoter, plasmid is built, realizes conditional gene knockout.It can realize fast and effective, simple and practicable, economical and efficient, the structure of conditional gene knockout animal model applied widely, the defects of can solving above-mentioned conventional method (specificity restructuring enzyme system Cre LoxP). |
priorityDate | 2014-12-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 28.