http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104745545-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P7-50 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0022 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y104-03011 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P7-50 |
| filingDate | 2015-04-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-02-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2018-02-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104745545-B |
| titleOfInvention | A kind of method of efficiently production L dglutamic oxidases |
| abstract | The invention discloses a kind of method of efficiently production L dglutamic oxidases, belong to Fermentation Engineering and technical field of enzyme engineering.25 30 DEG C of the optimal inductive condition of L dglutamic oxidases recombinant bacterium in the inventive method, lactose 5g/L inductions 5h；Studied by the different feed profile of fed batch fermentation, find to be advantageous to the increase of cell density by the way of index feed supplement and DO stat are combined；By the optimum induction to batch fermentation, it is logarithmic phase OD to find optimal inductive condition 600 =40,10g/L lactose induction 12h, enzyme activity are up to 156.1U/mL；Height is expressed into restructuring wet thallus to be added in the buffer solution of the glutamic acid pH8.0 containing 110g/L, 37 DEG C of conversion 24h can produce 107.9g/L alpha Ketoglutarate, and conversion ratio reaches more than 90%, and required bacterium solution amount is only the 1/50 of shake flask fermentation. |
| priorityDate | 2015-04-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 36.