http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104745544-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-26 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0006 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-26 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-04 |
| filingDate | 2015-03-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2017-10-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2017-10-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104745544-B |
| titleOfInvention | A kind of D LOs and its application in D lactate detections |
| abstract | The invention discloses a kind of D LOs, the amino acid sequence of the enzyme is soluble protein as shown in SEQ ID NO.2, and co-factor is FAD, and its optimum temperature is 55 DEG C, and optimal pH is 8.0, the enzyme activity in the range of pH 7.0~9.0 with more than 60%；The enzyme can be catalyzed D lactic acid oxidation generation pyruvic acid and hydrogen peroxide by direct electron acceptor of molecular oxygen, when D lactic acid concns are in the range of 0.1~1.3mmol/L, and the concentration of hydrogen peroxide of enzymatic generation is linearly related to initial D lactic acid concns；Apparent K of the enzyme to D lactic acid m And V max Respectively 3.62 ± 0.53mmol/L and 0.52 ± 0.05U/mg, the enzyme is to another substrate O 2 Apparent K m And V max Respectively 0.16 ± 0.01mmol/L and 0.97 ± 0.03U/mg.D LOs of the present invention can be used for preparing the biology sensor for detecting D lactic acid by determining concentration of hydrogen peroxide, realize that D lactic acid is easy, quickly quantitatively detect. |
| priorityDate | 2015-03-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 45.