http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104738028-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0735 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01N1-02 |
| filingDate | 2015-03-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2017-01-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2017-01-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104738028-B |
| titleOfInvention | A kind of stem cell quick-freezing method and the freezing liquid of use thereof |
| abstract | The invention provides a kind of stem cell quick-freezing method and the freezing liquid of use thereof, described stem cell quick-freezing method comprises the following steps: balanced in cryoprotective extender by stem cell；Go to stem cell No. 1 freezing liquid balances；After going to stem cell No. 2 freezing liquids balance, stem cell is transferred in support tube；Support tube keeps level near liquid nitrogen vapor layer, and carries out rotating to being vertically to liquid nitrogen liquid level to liquid nitrogen liquid level with support tube either end for the center of circle, make support tube is loaded with being partially submerged in liquid nitrogen of cell.Technical scheme can preferably protect stem cell when carrying out rapid glass freezing; it is effectively improved stem cell recovery survival rate after glass freezing, and the totipotency such as Growth and Differentiation and ability to express of keeping stem cell after Cryopreservation is unaffected；Especially for the stem cell that some are very sensitive, recovery survival rate can bring up to more than 95%. |
| priorityDate | 2015-03-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 25.