http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104730234-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_4f9d4a007fac37228f1455e94c2ccb9c |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5438 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-532 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N27-30 |
| filingDate | 2015-02-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b92d07e4a73c8b1ad32e191fd8f400e4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_285f6d4906ac73fa6eb4d25a010fbcbd http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_254a22814ceff752ac0dda01f8f44516 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e1854b6eeb3b6ee690d068556b6c0aad |
| publicationDate | 2015-06-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104730234-A |
| titleOfInvention | Preparation method and application of electrochemical sensing element based on immunogold |
| abstract | The invention discloses a preparation method of an electrochemical sensing element based on immunogold. A common nitroimidazole type structure hapten is prepared, and the nitroimidazole hapten is coupled with carrier proteins (BSA and OVA) by adopting an active ester method to prepare an immunogen and a coating antigen, wherein the mol ratio of coupling molecules is (11 to 1) and (10 to 1). A nitroimidazole antibody is simultaneously prepared and the valence is (1 to 100000), and immune nano gold is prepared; and a monomolecular film is formed on a gold electrode by using a monomolecular film self-assembling technology, and is connected with the immune nano gold through DCC and NHS to prepare the sensing element. The sensing element has the good responses on metronidazole, tinidazole and dimetridazole; the cross reaction rate is 90% or more; metronidazole, tinidazole and dimetridazole and metabolites of metronidazole, tinidazole and dimetridazole in a concentration range of 0.001-100mg/L can be determined; and the detection limit is about 0.0019mg/L and the recycling rate is about 90%. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106353381-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110296980-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110296980-B |
| priorityDate | 2015-02-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 95.