http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104726435-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y302-01021 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-42 |
| filingDate | 2015-04-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-05-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2018-05-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104726435-B |
| titleOfInvention | A kind of beta-glucosidase enzyme mutant, its recombinant expression plasmid and the engineered strain of conversion |
| abstract | The present invention obtains gene, mutant plasmid, the engineering bacteria for the β glucuroides that ethanol tolerance concentration improves by the method for site-directed point mutation, and can obtain the β glucuroides that ethanol tolerance concentration improves after by engineering bacteria induced expression.The present invention, by PCR rite-directed mutagenesises, obtains mutator based on the β glucuroides from ocean Anticipated transient without scram source.Under the conditions of 35 DEG C, versus wild type β glucoside zymoproteins, the ethanol tolerance concentration IC of the mutain of acquisition 50 1.8 times are improved, and glucose-tolerant concentration improves 2 times, pH and temperature stability improve, and substrate affinity and catalytic efficiency are promoted.The mutant can prepare aglycon by effectively hydrolyzing soybean isoflavone glucoside within a short period of time, have potential using value. |
| priorityDate | 2015-04-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 59.