http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104726406-B
Outgoing Links
Predicate | Object |
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classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-079 |
filingDate | 2015-02-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2018-05-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2018-05-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-104726406-B |
titleOfInvention | It is a kind of to induce the method that dental pulp Derived from Mesenchymal Stem Cells is nerve cell |
abstract | It is a kind of to induce the method that dental pulp Derived from Mesenchymal Stem Cells is nerve cell, dental pulp mescenchymal stem cell is extracted by enzyme digestion, it is inoculated into and has been coated with the culture dish of poly-D-lysine and laminin, addition Optimal Medium is placed in cultivating amplification under hypoxia condition;Then double-deck coating is carried out to the dental pulp mescenchymal stem cell of collection using de- cell amnion stroma, separately carries out induction differentiation using nerve cell induction liquid;Finally nerve cell long-term cultivation is maintained with nerve cell maintaining liquid.The present invention uses Optimal Medium and hypoxia condition culture, adds the quantity of dental pulp mescenchymal stem cell;Guiding role is played for the growth of nerve cell using double-deck de- cell amnion stroma analog neuron sheath structure, while is broken up using nerve cell induction liquid induction dental pulp mesenchymal stem cells into nerve, improves rate of differentiation;Neural cell activity is maintained for a long time using nerve cell maintaining liquid, and therapic opportunity is flexibly grasped easy to clinician. |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2021225552-A1 |
priorityDate | 2015-02-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 256.