http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104711259-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-84 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H5-00 |
| filingDate | 2015-03-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2017-12-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2017-12-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104711259-B |
| titleOfInvention | A kind of double miRNA suppress expression vector and its construction method and application |
| abstract | The invention discloses a kind of double miRNA to suppress expression vector and its construction method and application, and the present invention reacts by the overlapped extension PCR of primer of Seq1 and Seq2, obtains the sequence Seq3 of 8~10 Seq1 repeated arrangements.Seq3 sequence fragments are connected on pEASAY T1 cloning vectors, obtain cloning vector pEASAY T1 Seq3, sequence fragment Seq3 is connected further on plant expression vector pBIGFP after digestion, obtain double miRNA and suppress expression vector pBIGFP Seq3, double miRNA of the present invention suppress expression vector and play the effect for suppressing clover Ms miR393 and Ms miR398 activity. |
| priorityDate | 2015-03-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 28.