http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104651499-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6851 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6888 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6851 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
| filingDate | 2015-01-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-10-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2018-10-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104651499-B |
| titleOfInvention | The method for carrying out gene function verification for panonychus citri |
| abstract | The present invention relates to a kind of methods carrying out gene function verification for panonychus citri, include the following steps：1) prepare target gene dsRNA solution：Corresponding dsRNA solution is synthesized according to the target gene to be inhibited；2) leaf dish is made with Citrus leaf, cleaned up；3) leaf dish is dried to the apparent shrinkage of leaf dish, the dsRNA solution prepared in step 1 is contained with centrifuge tube lid, leaf dish is floated on into dsRNA solution up to the no longer shrinkage that restores to the original state；4) panonychus citri is placed on the leaf dish of floating, leaf dish is put into incubator, in 22-28 DEG C, humidity 55-65% and illumination:Dark is 14h:It is cultivated 60-80 hours under conditions of 10h；5) it after cultivating, collects panonychus citri polypide and extracts RNA, the expression of the target gene in detecting step 1.The present invention solves the problems, such as panonychus citri currently without effective dsRNA introduction methods. |
| priorityDate | 2015-01-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 28.