http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104640985-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-56 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-55 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-622 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-28 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1055 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1082 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1241 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6854 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-10 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-90 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
| filingDate | 2013-07-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-05-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-05-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104640985-B |
| titleOfInvention | Transposition-mediated identification of specific binding or functional proteins |
| abstract | The methods disclosed herein describe a novel technology for exploring and optimizing polypeptides (including antigen binding molecules, such as antibodies and fragments thereof) having a desired binding specificity and/or functionality to obtain a desired functional and/or binding phenotype that is not heretofore efficient, flexible, practical, and high-speed. The new method is based on: transposable structures and diverse DNA libraries cloned into transposable vectors, and their transfection into host cells by co-transient expression of functional transposases. This ensures efficient and stable one-step introduction of transposon-based expression vectors into vertebrate host cells, followed by screening of the cells for the desired functional or binding phenotype of the expressed protein, which can then be identified by standard cloning and DNA sequencing techniques for the relevant coding sequences of the expressed protein, including antibodies and fragments thereof. |
| priorityDate | 2012-07-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 88.