http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104614459-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-02 |
| filingDate | 2015-01-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2016-09-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2016-09-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104614459-B |
| titleOfInvention | A kind of method of rapid enzymolysis glycosylated albumin |
| abstract | A kind of method that the invention discloses rapid enzymolysis glycosylated albumin, it is completed by following steps: 1) preparation is containing ionic liquid, protein denaturant, Proteinase K, CaCl 2 , Macrogol 6000, TritonX 100, the pH7.5 8.5Tris HCl buffer solution of Sodium azide；2) the glycosylated albumin sample liquid of the accurate definite value of addition HPLC method, reacts a period of time；3) in the solution of step (2) gained, EDTA 2Na solution, enzymolysis reaction are added；4) by glycosylated albumin concentration in HPLC method determination step (3) gained solution, glycosylated albumin enzymatic hydrolyzation is calculated.Carry out the enzymolysis of glycosylated albumin sample by this method, by the glycosylated albumin rapid enzymolysis of 300 900 μm ol/L in may be implemented in 180 600 seconds, be particularly suitable for FAOD method and measure the methodology of glycosylated albumin and measure reagent exploitation. |
| priorityDate | 2015-01-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 43.