http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104611457-B
Outgoing Links
Predicate | Object |
---|---|
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-686 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6806 |
filingDate | 2015-03-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2019-05-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2019-05-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-104611457-B |
titleOfInvention | Arm-PCR primer design method for multiple target point gene magnification |
abstract | The invention discloses a kind of Arm-PCR primer design methods for multiple target point gene magnification, through the following steps that realize: a, the primer that icubate2.0 online software design small fragment target gene is replaced with LAMP software;B, the cyclic primer sequence before inner primer that LAMP software design goes out is changed the universal linker sequence of Arm-PCR primer into;C, the preparation of Arm-PCR reaction system;D, the setting and operation of Arm-PCR reaction condition;E, the capillary electrophoresis detection of Arm-PCR product.The present invention is more using the primer sets that LAMP software design goes out, be conducive to the screening of multi-primers group, the present invention is used for the Arm-PCR primer design method of multiple target point gene magnification, it can enable the Arm-PCR primer successful design of small fragment target gene, and then Arm-PCR multiple reaction is made to become simple possible. |
priorityDate | 2015-03-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 19.