http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104611404-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-28 |
| filingDate | 2015-01-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2016-08-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2016-08-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104611404-B |
| titleOfInvention | Identify Winter Cole-wort and the method for juncea winter rape cenospecies |
| abstract | The present invention relates to Winter Cole-wort and the authentication method of juncea winter rape cenospecies.Utilizing the method successful identification Winter Cole-wort and juncea winter rape cenospecies is true hybrid.A kind of method identifying Winter Cole-wort and juncea winter rape cenospecies, it is characterized in that including following steps: (1) samples: after (2) weigh 0.2 0.3g alabastrum, flower, angle fruit respectively, respectively with the phosphate buffer of pH7.8, containing 2mmolASA, 5mmol EDTA Na 2 Being homogenized under ice bath, under 12000r/min, centrifugal 10min, takes supernatant and saves backup in 20 DEG C of refrigerators.Using polyacrylamide gel electrophoresis, separation gel and concentration gum concentration to be respectively 7% and 4%, pH value is respectively 8.8 and 6.8；Electrode buffer Tris glycine system, pH value 8.3, applied sample amount is 30 μ L；When electrophoresis starts, voltage stabilizing 100v, sample is modulated into 200v, electrophoresis time about 5h after crossing concentration glue；Electrophoresis is put into after completing in dyeing liquor benzidine solution and is dyeed, until visible blue bands clearly, after flowing water rinses, photograph preserves；Mobility Rf value is initial point to the migration distance/initial point of band to the distance of indicator. |
| priorityDate | 2015-01-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 31.