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filingDate 2014-12-30-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2017-11-17-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2017-11-17-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-104611305-B
titleOfInvention The Aflatoxin-detofizyme that trypsin-resistant improves
abstract The invention discloses a kind of Aflatoxin-detofizyme improved to trypsin-resistant.The present invention is transformed the critical amino acid residues at wild type Aflatoxin-detofizyme three-dimensional structure outer layer position by protein engineering, there is provided a kind of to obtain the ADTZ mutant improved to trypsin-resistant.Mutant Aflatoxin-detofizyme (the ADTZ of the present invention for acting on aflatoxin K221C/K252Q/K278S ), 2.73 times are improved than wild type ADTZ to the resistance of trypsase, its half-life period extends 72 minutes than wild-type enzyme, and other zymologic properties and wild-type enzyme are basically identical.
priorityDate 2014-12-30-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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