http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104603290-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2561-108 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2521-325 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6827 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-683 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6827 |
| filingDate | 2013-06-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-02-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-02-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104603290-B |
| titleOfInvention | Nuclease Protection Method for Detection of Nucleotide Variations |
| abstract | Disclosed herein are methods for detecting the presence of nucleotide variations in target nucleic acids using nuclease protection assays. The method comprises contacting a sample with at least two probes under conditions sufficient to hybridize the probe to the target nucleic acid, wherein the first probe is to a wild-type (non-variant) nucleoside at the position of the nucleotide variation in the target nucleic acid Acid complementation, the second probe is complementary to the variant nucleotide at the position of the nucleotide variant in the target nucleic acid, resulting in a mixture of hybridized and unhybridized nucleic acids. The mixture is contacted with a nuclease specific for single-stranded nucleic acid molecules under conditions sufficient to remove unhybridized nucleic acid molecules (or unhybridized portions of nucleic acid molecules). The presence of the at least two probes is then detected, thereby detecting the presence of variant and/or non-variant target nucleic acids in the sample. |
| priorityDate | 2012-06-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 290.