http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104585027-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H4-00 |
| filingDate | 2014-12-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2016-06-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2016-06-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104585027-B |
| titleOfInvention | A kind of hardening off method of Rhizoma Zingiberis Recens tissue cultured seedling |
| abstract | The hardening off method of a kind of Rhizoma Zingiberis Recens tissue cultured seedling of disclosure, comprises the following steps: (1) select healthy and strong, be tissue culture's material without the Rhizoma Zingiberis Recens of pest and disease damage, be embedded in sand bed accelerating germination after being sterilized by Rhizoma Zingiberis Recens block, sterilizing, the stem apex stripping 0.3～0.5mm is inoculated；(2) stem apex is passed sequentially through the cultivation of Aseptic seedling culture base, inducing culture, proliferated culture medium, root media, forms the tissue-culture container seedling that can go out culturing room；(3), when the tissue cultured seedling length in culture bottle has 4～5 Bletilla striata (Thunb.ex A.Murray)Rchb.f., lid seedling exercising is taken off；After 5 days, culture bottle is removed culturing room, condition lower refining seedling 7 days in natural lighting and temperature 24～26 DEG C, treat that 5～6 leaves occurs in tissue cultured seedling, during leaf length 5～7cm, in culture bottle, take out tissue cultured seedling and clean culture medium, being transplanted into the peat composed of rotten mosses: perlite: in the substrate of Vermiculitum=2:1:1, rich water quality management is to growing up to finished product Seedling routinely, goes out garden.The method is simple, and cost is low and transplanting survival rate is high. |
| priorityDate | 2014-12-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 142.