http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104561279-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-156 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6888 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2014-12-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2017-01-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2017-01-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104561279-B |
| titleOfInvention | Method of improving quality of chicken semen, primer used for method, kit and using method of kit |
| abstract | The invention discloses a primer for a gene closely related to the quality of chicken semen. The primer is prepared from the following components including a mixture of upstream and downstream primers PCR amplified by a gene of a vasoactive intestinal peptide receptor 1 (VIPR1), a mixture of upstream and downstream primers PCR amplified by a gene of a vasoactive intestinal peptide receptor 2 (VIPR2) and a mixture of upstream and downstream primers PCR amplified by a gene of a dopamine receptor 2 (DAR2). A method of improving the quality of the chicken semen comprises the following steps: during breeding screening, extracting DNA of a chicken genome of a sample to be tested, carrying out specific primer amplification on a gene sequence to obtain target fragments of 192, 250 and 270 bp, after SSCP analysis and gel electrophoresis on the target fragments, coloring the target fragments by virtue of silver staining to obtain DNA and SSCP maps, and selecting a genotype individual of three genes of TT/GG/OQ according to banding pattern selection in three maps. A kit comprises mixtures of upstream and downstream primers PCR amplified by genes of the VIPR1, the VIPR2 and the DAR2 and a reagent needed by PCR. A using method of the kit comprises the following steps: carrying out PCR amplification on a fragment located by an SNP site; carrying out PCR-SSCP detection; and carrying out SSCP analysis. The method is remarkable in effect and simple and rapid in detection method and is not influenced by external environment. |
| priorityDate | 2014-12-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 33.