http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104561089-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-84 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H5-00 |
| filingDate | 2014-12-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2017-07-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2017-07-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104561089-B |
| titleOfInvention | A kind of breeding method of Transgenic melon tissue-cultured seedling and application |
| abstract | The invention discloses a kind of breeding method of Transgenic melon tissue-cultured seedling and application, belong to field of plant genetic.Method provided by the present invention for muskmelon seedses are shelled, sterilized, soak after cultivated, obtain transformation receptor, by containing target gene recombinational agrobacterium activation, it is resuspended, before cotyledon is not deployed, remove the terminal bud and lateral bud of transformation receptor, the recombinational agrobacterium containing target gene is injected at cotyledonary node separate living tissue, expand adventitious bud at induction muskmelon shoot tip meristem differentiation, bud elongation growth, which is obtained, infects Muskmelon Seedlings, it is transplanted in soil and is cultivated, finally utilizes kanamycins screening transgenic plant.The method of the present invention does not need the sterile working of culture medium, the adventitious bud of generation is more, high conversion rate is up to 16 20%, limited small by muskmelon genotype, transformation period is short, being infected from plant to the contemporary seed of harvest is only needed 34 months, and performing PCR detection can be entered after 20 30d, plenty of time, personnel, financial resources, material resources are saved. |
| priorityDate | 2014-12-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 34.