http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104560743-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12R2001-84 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-165 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-16 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-84 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-16 |
| filingDate | 2013-10-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-09-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2018-09-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104560743-B |
| titleOfInvention | A kind of pichia farinose high cell density fermentation method |
| abstract | The invention belongs to field of agricultural microbial technology, and in particular to a kind of pichia farinose high cell density fermentation method, including seed prepares and fermentation tank culture.Fermentation tank culture condition：Liquid amount 40%~60%（V/V）, inoculum concentration 1%~5%（V/V）, 28~40 DEG C, 1~3.5VVM of ventilatory capacity of temperature is controlled, rotating speed 300r/min is originated, rotating speed 100r/min is improved every 4h, improves to 700r/min and remain unchanged, 20~30h of fermentation period.It is 6.0 or so to adjust zymotic fluid pH by dropwise addition NaOH solution in fermentation process.Ferment a certain amount of glucose of intermittent injecting after 13h, and fermentation ends after 20~30h, living bacteria count is 30.0 35.0 hundred million/mL.Fermentation process using the present invention, not only biomass obviously increases substantially, but also concise in technology, efficiency are lower better than the prior art, production cost. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109321477-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109321477-A |
| priorityDate | 2013-10-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 36.