http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104542301-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H4-00 |
| filingDate | 2015-01-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2015-12-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2015-12-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104542301-B |
| titleOfInvention | A kind of method of excised cotyledon osmund |
| abstract | The method of excised cotyledon osmund, clip osmund fertile spike top immature green spores capsule fringe; Explant sterilization <b>, </b> osmund fertile spike is cut into chunks, and is inoculated in agar medium and cultivates; The young prothallium sprouted is transferred in prothallium propagation agar medium, temperature 23-26 DEG C, illumination 12h/d intensity 2300-2500? lx; Breed prothallium taking-up and put into the aseptic triangular flask that liquid nutrient medium is housed, medium 1/2MS+6-BA in Spore cultivation? 0.1mg/L+NAA? 0.01mg/L, shaking table vibrates 4-10min, getting appropriate prothallium access is respectively placed with in the blake bottle of filter paper bridge or cotton, pour MS liquid nutrient medium into, cultivate; Sprout sporophyte to transfer in the blake bottle being placed with filter paper bridge or cotton, pour sporophyte propagation liquid nutrient medium into; Cultivate. |
| priorityDate | 2015-01-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 23.