http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104531762-B
Outgoing Links
Predicate | Object |
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classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-867 |
filingDate | 2014-12-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2017-06-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2017-06-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-104531762-B |
titleOfInvention | The slow virus carrier and its construction method and application of a kind of RAB7A and EGFP gene overexpression fusion green fluorescent protein |
abstract | The invention provides a kind of RAB7A genes and EGFP gene overexpression fusion green fluorescent protein slow virus carrier and its construction method and application.Carrier of the present invention is with slow virus carrier LV11 as skeleton carrier, behind the CMV promoter of LV11 carriers EGFP gene and RAB7A genetic fragments are inserted by 5 ' to 3 ' directions, the fusion green fluorescent protein slow virus carrier of the EGFP and RAB7A gene overexpressions that the present invention builds, the problems such as solving in RAB7A gene studies over-express vector effectively and be directly perceived.The carrier ensure that eukaryotic gene high efficient expression with CMV as promoterï¼›It is label to use EGFP, the effect of the degree and RAB7A genes of cell generation autophagy and endocytosis during cell hypoxia can be judged by the power of observation expression fluorescence directly perceived, for the function of studying RAB7A genes provides good instrument, for the correlative study of follow-up RAB7A provides basis. |
priorityDate | 2014-12-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 69.