http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104530262-B
Outgoing Links
Predicate | Object |
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classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C08B37-10 |
filingDate | 2015-01-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2016-08-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2016-08-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-104530262-B |
titleOfInvention | A kind of production method extracting heparin from Pulmonis Sus domestica |
abstract | A kind of production method extracting heparin from Pulmonis Sus domestica, step is as follows: fresh Pulmonis Sus domestica rubs into serosity, serosity insulation enzymolysis, enzymolysis solution are collected by filtration filtrate, filtrate carries out removing protein process, ultrafiltration, oxidation precipitation, lyophilization obtain heparin crude product.Advantages of the present invention and have the beneficial effect that 1, enzyme solution that the present invention uses more thoroughly can dissolve Pulmonis Sus domestica serosity all sidedly, enzymolysis is terminated with trypsin, Vacuum Freezing & Drying Technology stabilizes the quality of product and improves yield, and the crude product impurity simultaneously obtained is few, titer is high;2, production method of the present invention uses clarifier to substitute ethanol precipitate with ethanol repeatedly to make a return journey the traditional handicraft of isolating protein, there is clearance high, saved the usage amount of a large amount of ethanol, reduced production cost;3, present invention process is reasonable in design, produces nontoxic, harmless, biological green environmental protection, and Pulmonis Sus domestica raw material sources are easy to get, operation low cost, is suitable for large-scale industrial production. |
priorityDate | 2015-01-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 42.