http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104515817-B

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Predicate Object
classificationIPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-02
filingDate 2014-11-17-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2017-01-11-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2017-01-11-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-104515817-B
titleOfInvention Method for evaluating destroy degree on nucleotide of determination method and production technology of free nucleic acid hydrolysate in protein product
abstract The invention discloses a method for determining a free nucleic acid hydrolysate in protein products. The method comprise the following steps: (1) respectively preparing a nucleotide mixed standard solution and a bases mixed standard solution; (2) preparing a solution of a sample to be tested, and using the nucleotide mixed standard solution and the base mixed standard solution described in step 1 as the reference solution, and employing high performance liquid chromatography through washing peak area method comparison to obtain the content of free nucleotides and free base in the sample. According to the invention, high performance liquid chromatography is used for determining 5 kinds of bases in a single-cell protein raw material (cytosine, uracil, guanine, thymine and adenine), 5 kinds of nucleotides (cytidine monophosphate, uridylic acid, guanylic acid, inosinic acid and adenylic acid), and 5 kinds of nucleosides (cytidine, uridine, inosine, guanosine and adenosine); and then the destroy degree on nucleotide of the production process is determined according to t the relationships between the bases and nucleotides, and between the bases and nucleosides.
priorityDate 2014-11-17-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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