http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104515758-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 |
| filingDate | 2014-12-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2017-12-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2017-12-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104515758-B |
| titleOfInvention | Promote the quantitative detecting method of cancer activity and the screening technique of carcinogenic promoting agent |
| abstract | A kind of method for the rush cancer activity for quantitatively detecting determinand, comprises the following steps：Suitable cell model is selected, is divided into donorcells and recipient cell as desired；Add two kinds of fluorescence probes simultaneously in donorcells and be incubated；Donorcells and recipient cell are mixed in proportion；Determinand is added in tested group of mixed culture cell, control group does not add；After being incubated certain time, flow cytometry measures single positive cell number that a kind of fluorescence probe in tested group and control group donorcells is transferred to recipient cell；Two groups of Te values (transfer efficiency, Transfer effects) are calculated, use Te respectively con And Te test Represent, Te values=mono- positive cell number/donorcells number × 100%；Use Te R Represent the Te relative values of the determinand, Te R =Te test /Te con × 100%；According to the Te of determinand R Evaluate the rush cancer activity size of determinand, Te R It is smaller show the determinand rush cancer activity it is stronger.Further relate to the above method and screen application in carcinogenic promoting agent in vitro, work as Te R During less than 90%, carcinogenic promoting agent is accredited as. |
| priorityDate | 2014-12-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 232.