http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104513861-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-13 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-158 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6895 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6844 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6895 |
| filingDate | 2015-01-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-03-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-03-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104513861-B |
| titleOfInvention | LAMP detection primer group, detection kit and the detection method of genetically engineered soybean MON89788 and its derived varieties |
| abstract | The invention discloses a kind of genetically engineered soybean MON89788 and its LAMP detection primer group, detection kit and the detection methods of derived varieties.Detection primer group includes four specific primers；Detection kit includes primer liquid, reaction solution, archaeal dna polymerase and control；Kit can also have color developing agent or fluorescence indicator；Its detection method be by extracting the DNA of soybean varieties to be checked, using four specific primers and a kind of archaeal dna polymerase with strand-displacement activity, sample DNA templates are expanded at 60~65 DEG C, short time amplification efficiency can reach 10 9 ~10 10 A copy is identified and uses the turbidity precipitated in reaction tube variation, color change in color developing agent observing response pipe is added, observes whether determining soybean varieties to be checked contain or for genetically engineered soybean MON89788 and its derived varieties using real-time fluorescence detector.The present invention have many advantages, such as rapidly and efficiently, easy to operate, high specific, high sensitivity, identification is easy, is suitble to on-site test, be suitable for popularization and application. |
| priorityDate | 2015-01-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 40.