http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104513306-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K38-00 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-775 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K38-17 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P9-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-775 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-20 |
| filingDate | 2014-12-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2016-08-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2016-08-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104513306-B |
| titleOfInvention | The purification process of Apolipoprotein A1 and ApoAI protein injection antigen |
| abstract | The invention provides the purification process of a kind of Apolipoprotein A1, including: obtain plasma sample, and carry out clarifying treatment；Plasma sample after clarification is carried out hydrophobic chromatography for the first time, obtains purified product for the first time；First time purified product is carried out ungrease treatment；First time purified product after ungrease treatment is carried out second time hydrophobic chromatography；Wherein, the eluting of purification includes twice eluting for the first time；Eluting carries out eluting, for the second time eluting alcohol of 25～30% with the Organic Alcohol of 5～10% and carries out eluting for the first time；For the second time hydrophobic chromatography by eluotropic strength less than 25～the elution of alcohol of 30%.The method of the present invention utilizes the mechanism that there is the strong-hydrophobicity that lipid produces in HDL structure to carry out purification for the first time, then the lipoprotein structure destroying HDL makes Apolipoprotein A1 separate with lipid, cause Apolipoprotein A1 with as high hydrophobicity heteroproteins hydrophobic difference increase after the most hydrophobic separate, i.e. obtain the preferable Apolipoprotein A1 of purity；Method simplicity is novel, the amount of process sample is bigger, efficiency is high. |
| priorityDate | 2014-12-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 126.