http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104498591-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6827 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2014-11-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2016-06-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2016-06-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104498591-B |
| titleOfInvention | Method based on liquid-phase chip detection by quantitative Alu gene methylation level |
| abstract | The invention discloses a kind of method based on liquid-phase chip detection by quantitative Alu gene methylation level, the Alu sequence after converting for sodium sulfite including (1) designs corresponding primer；(2) specimen serum CRP extracts and sodium sulfite process；(3) specimen after processing is carried out BSP order-checking；(4) according to sequencing result, Analysis and Screening goes out detection site, and designs correspondent probe for this site；(5) standard substance and sample P CR product are prepared；(6) probe is coated on microsphere (7) microsphere of probe and PCR primer hybridization incubation will be coated, and detect on liquid-phase chip machine, read data；(8) according to after standard substance drawing standard curve, the methylation level of each sample is calculated.The inventive method, compared to existing detection method, is a kind of high flux, method easy and simple to handle, detection by quantitative Alu gene methylation level accurately and reliably. |
| priorityDate | 2014-11-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 46.