http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104498516-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P3-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-63 |
| filingDate | 2014-11-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-05-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2018-05-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104498516-B |
| titleOfInvention | Highly effective hydrogen yield functional gene carrier pETD-SL and its structure and application |
| abstract | The invention discloses a kind of highly effective hydrogen yield functional gene carrier pETD SL, and its construction method and application, it is related to gene engineering technology field.PETD SL genophores are 1 plasmids of pETDuet for including [NiFe] hydrogenase gene.Its structure is the large subunit hupL and small subunit hupS genes by designing primer amplification [NiFe] hydrogenase gene fragment, and it is successively connected to also correspond in 1 fragments of pETDuet for connecting end above-mentioned two genetic fragment with digestion with restriction enzyme and with DNA ligase, gene sequencing is carried out, verifies the integrality of gene.PETD SL plasmids are transformed into bacterium, this bacterium production hydrogen is cultivated in hydrogen culture medium is produced.PETD SL can be applied in different bacterium, can make the Hydrogen Production by Bacteria for not producing hydrogen, the hydrogen generation efficiency of the bacterium of production hydrogen can also significantly improved, and hydrogen faster and more can be produced by consuming same concentration of substrate.Also the Escherichia coli of existing industry production hydrogen can be improved. |
| priorityDate | 2014-11-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 24.