http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104483178-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N1-30 |
| filingDate | 2014-11-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-03-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2018-03-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104483178-B |
| titleOfInvention | The preparation method of epinephelus akaara adult fish chromosome |
| abstract | A kind of preparation method of epinephelus akaara adult fish chromosome, belongs to cell biology, and it includes material processing early stage, prepares renal cell suspension, Hypotonic treatment, fixation, drop piece and dyeing；Described material processing early stage is using phytohaemagglutinin internal injection method, by the dosage of 15 μ g/g fish bodies weight, PHA is injected to the pectoral fin base portion of experiment fish, weighs the dosage injection colchicine solution of 2 μ g/g fish bodies weight after 24h by fish body, after 2.5 3h, head-kidney is taken out.The present invention uses the ground method of shot phytohaemagglutinin, while increases injection concentration, just can obtain good effect after 24h, save the time.The present invention is hardly resulted in complete cell, therefore the change prior art of the invention when carrying out Hypotonic treatment by prior art, is obtained clearly chromosome picture due to the histocyte characteristic of epinephelus akaara. |
| priorityDate | 2014-11-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 17.