http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104480215-B

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classificationCPCAdditional http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-156
classificationCPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6886
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844
classificationIPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11
filingDate 2014-12-30-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2016-12-07-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2016-12-07-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-104480215-B
titleOfInvention A kind of gene association detection method and test kit
abstract A kind of method that the present invention relates to joint-detection gene mutation, with 20-50ng DNA as sample, detects KRAS, PIK3CA, BRAF and EGFR gene 16 kinds of sudden changes altogether simultaneously.The method comprise the steps that design specific probe, set up MLPA amplification mutant gene sequence reaction system, react a large amount of amplifying target genes sequence by PCR.High specificity of the present invention, the wild type DNA of 10~100ng will not produce nonspecific interference signal;Selectivity is strong, can 99~99.9% wild type DNA background under detect the saltant type of 0.1%~1.0%.
priorityDate 2014-12-30-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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