http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104480215-B
Outgoing Links
Predicate | Object |
---|---|
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-156 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6886 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 |
filingDate | 2014-12-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2016-12-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2016-12-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-104480215-B |
titleOfInvention | A kind of gene association detection method and test kit |
abstract | A kind of method that the present invention relates to joint-detection gene mutation, with 20-50ng DNA as sample, detects KRAS, PIK3CA, BRAF and EGFR gene 16 kinds of sudden changes altogether simultaneously.The method comprise the steps that design specific probe, set up MLPA amplification mutant gene sequence reaction system, react a large amount of amplifying target genes sequence by PCR.High specificity of the present invention, the wild type DNA of 10~100ng will not produce nonspecific interference signal;Selectivity is strong, can 99~99.9% wild type DNA background under detect the saltant type of 0.1%~1.0%. |
priorityDate | 2014-12-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 77.