http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104480090-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-4727 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-6481 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-47 |
| filingDate | 2014-11-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2017-05-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2017-05-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104480090-B |
| titleOfInvention | Preparation method of porcine pepsin and gastric mucin |
| abstract | The invention discloses a preparation method of porcine pepsin and gastric mucin, and the preparation method is designed for achieving the purpose of overcoming the defects that by using traditional pepsin preparation methods, pepsin is easily degenerated, organic solvents hurts the bodies of operators, and the cost is high, and the like. The method comprises the following steps: carrying out crude extraction on the mucosa of a basilar part of a porcine stomach by using a hydrochloric acid; then, by using the properties that pepsin is high in solubility and stable in enzyme activity in a weakly acidic solution, and gastric mucin is precipitated after meeting a weak acid, extracting and separating porcine pepsin and gastric mucin by using an acidic aqueous solution replacing acetone; and carrying out centrifugation, filtration, ultrafiltration, drying, and the like on the obtained product, so that porcine pepsin and gastric mucin are obtained. In case that the method is adopted for preparing porcine pepsin and gastric mucin, the activity of pepsin is protected, and meanwhile, because no organic solvent is used, the safety factor of operating is improved, and no harm is caused to the bodies of workers; and the cost is reduced, and the production capacity is increased, so that the method has broad market prospect. |
| priorityDate | 2014-11-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 59.