http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104480054-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12R2001-01 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-03 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N13-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-01 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-205 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-01 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N13-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-03 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-01 |
| filingDate | 2014-10-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-07-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2018-07-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104480054-B |
| titleOfInvention | A kind of bacterial strain and preparation method thereof of acid resistance high yield succinic acid |
| abstract | The invention discloses a kind of bacterial strains and preparation method thereof of acid resistance high yield succinic acid, after the critical pH for determining Actinobacillus succinogenes ATCC55618 strain growths is 4.5, ultraviolet mutagenesis is carried out to it, filter out the bacterial strain M1 that can be grown on the solid plate of pH4.3, the ultraviolet nitrosoguanidine complex mutation of two-wheeled is carried out to M1, filters out bacterial strain for high yield of succinic acid AS R2, which can be with growth and breeding under the conditions of pH4.1, but under the conditions of pH5.0, it is only 16.54g/L to produce acid amount.The present invention carries out 3 wheel gene shufflings using genome segment S9 technology to AS R2, obtains acid resistance superior strain AS F3 2, can pH3.2 under the conditions of growth and breeding, under the conditions of pH5.0, ferment 36h, and production succinic acid production reaches 25.2g/L. |
| priorityDate | 2014-10-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 71.