| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_4fcbe99de2e1bc092edc299b68dac02b |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12R2001-01 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N13-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-01
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-205
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-03 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-01 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-03
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-01
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N13-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 |
| filingDate |
2014-10-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5c2ea0cfa12faea00b503a03cbf3d70c
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_66c4d05e64ed1a0c91a230d87173f667
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bd92ff7a40e249a10b1683f8b5f63077
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bccaffbbf60eb84d818a6cee6aad04ed
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b521738ebeadd4f08fa2e05042dc2fce
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c16ed7b536c15e764fb5ac59a21a0806
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8934323d8b3eec3ab0423fadbb7b8b6e
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_57259586fa55f883248fc051517dbb12 |
| publicationDate |
2015-04-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CN-104480054-A |
| titleOfInvention |
Acid resistance and high succinic acid yield strain and preparation method thereof |
| abstract |
The invention discloses an acid resistance and high succinic acid yield strain and a preparation method thereof. According to the present invention, after the critical growth pH of an Actinobacillus succinogenes strain ATCC55618 is determined as 4.5, ultraviolet mutagenesis is performed on the strain, the strain M1 capable of growing on a solid flat plate with the pH value of 4.3 is screened, and two rounds of ultraviolet-nitrosoguanidine composite mutagenesis are performed on the M1 to screen the high succinic acid yield strain AS-R2, wherein the high succinic acid yield strain AS-R2 can grow and breed under the condition of pH4.1, and the acid yield is only 16.54 g/L under the condition of pH5.0; and the three rounds of genome re-organizations are performed on the AS-R2 by using the genome re-organization technology to obtain the acid resistance and high succinic acid yield strain AS-F3-2, wherein the acid resistance and high succinic acid yield strain AS-F3-2 can grow and breed under the condition of pH3.2, and the succinic acid yield is 25.2 g/L after fermentation for 36 h under the condition of pH5.0. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109306348-A |
| priorityDate |
2014-10-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |