http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104472351-B
Outgoing Links
Predicate | Object |
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classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H4-00 |
filingDate | 2014-11-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2016-08-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2016-08-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-104472351-B |
titleOfInvention | A kind of method of in vitro Qaidam matrimony vine not final singling induction |
abstract | The method that the present invention relates to the matrimony vine not final singling induction of a kind of in vitro Qaidam, the method comprises the following steps: takes the most annual 5 ~ July and gives birth to spray then in the excellent strain of Qaidam matrimony vine, and blade is removed, after rinsing well with flowing water, by the branch section of cutting post rinse, sterilizing i.e. obtains aseptic stem section;(2) aseptic stem section is seeded in pre-culture medium cultivation as outer implant, obtains the outer implant of pre-incubated stem section;The outer implant of the most pre-incubated stem section is transferred and is cultivated in the induction of the lateral bud culture medium, the lateral bud that must be newly formed;(4) the lateral bud being newly formed is cut in not final singling differential medium of transferring and cultivate, after 3 ~ 4 weeks, i.e. obtain not final singling;(5) the not final singling that will grow to 1 ~ 2cm proceeds to proliferated culture medium cultivation 4 weeks.The present invention is simple to operate and can keep maternal character. |
priorityDate | 2014-11-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 35.