http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104471075-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-686 |
| filingDate | 2013-03-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-06-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2018-06-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104471075-B |
| titleOfInvention | nucleic acid amplification |
| abstract | In some embodiments, the present teachings provide methods for nucleic acid amplification comprising forming a reaction mixture, and subjecting the reaction mixture to conditions suitable for nucleic acid amplification. In some embodiments, methods for nucleic acid amplification include subjecting nucleic acid to be amplified to partially denaturing conditions. In some embodiments, methods for nucleic acid amplification include performing amplification without fully denaturing the amplified nucleic acid. In some embodiments, methods for nucleic acid amplification use enzymes and polymerases that catalyze homologous recombination. In some embodiments, methods for nucleic acid amplification can be performed in a single reaction vessel. In some embodiments, methods for nucleic acid amplification can be performed in a single continuous liquid phase of a reaction mixture without compartmentalization of the reaction mixture or immobilization of reaction components. In some embodiments, a method for nucleic acid amplification comprises amplifying at least one polynucleotide on a surface under isothermal amplification conditions, optionally in the presence of a polymer. The polymer may include sieving agents and/or diffusion reducers. |
| priorityDate | 2012-04-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 891.