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filingDate 2012-07-17-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2016-10-05-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2016-10-05-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-104471065-B
titleOfInvention The gene coming from the grand genome of mud flat and the protein demonstrating phospholipase and lipase activity altogether obtained from it
abstract The present invention relates to come from the new gene of the grand genome of mud flat, and relate to the novel protein demonstrating phospholipase and lipase activity altogether obtained from it.Especially, the new gene separated from the grand genomic library of tidal flat soil microorganism and the described protein with phospholipase and lipase active encoded by described new gene: express with water-soluble form, therefore can produce in a large number;The protein of ultra-high purity it is obtained in that by the single step purification using Ni NTA post;PH5 to 10 in the range of show good activity;Good low temperature active and stability is maintained at temperature reaches 3 DEG C to 40 DEG C;And have for multiple organic solvent is resistance.Therefore, described new gene and protein can be usefully used for purification and conversion, biomedicine and the fine chemistry of multiple industrial circle, such as oil & fat.
priorityDate 2011-11-18-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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