http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104450880-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6858 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6858 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 |
| filingDate | 2014-10-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-06-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2018-06-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104450880-B |
| titleOfInvention | A kind of primer and method of the single nucleotide polymorphism for detecting goat DQA1 gene extrons 4 |
| abstract | The invention discloses a kind of primers and method of the single nucleotide polymorphism for detecting goat immune related gene DQA1 genes, primer is made of sense primer and anti-sense primer, using goat genomic DNA as template during detection, PCR reaction amplification target fragments are carried out using upstream and downstream primer, PCR product is handled with denaturant, native polyacrylamide gel electrophoresis detection is carried out to the amplified fragments after denaturation, glue is contaminated using argentation, is that can determine that the allele of goat DQA1 gene extrons 4 according to polyacrylamide gel electrophoresis result.The nucleotide primer and the detection architecture of foundation designed by the present invention more can accurately and fast expand different allelotypes in group, can accurately analyze to obtain the different single nucleotide mutation sites of goat DQA1 gene extrons 4. |
| priorityDate | 2014-10-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 30.