http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104450784-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-85 |
| filingDate | 2014-11-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2017-03-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2017-03-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104450784-B |
| titleOfInvention | Method for establishing SAMHD1 gene knockout cell line |
| abstract | The invention discloses a method for establishing an SAMHD1 gene knockout cell line. The method comprises the following steps: totally designing four pairs of recognition sequences aiming at SAMHD1 genes CDS1 and CDS2; respectively connecting gene segments of a target recognition unit module in series according to the four pairs of recognition sequences, cloning the gene segments into pCAG-T7-TALEN (Sangamo)-Destination plasmid, and establishing four pairs of TALEN expression plasmid pairs; and respectively transfecting 293T cell with the four pairs of TALEN plasmid pairs, and screening a cell line with the SAMHD1 genes knocked out by using neomycin. According to the method, frameshift mutation can be caused at target sites of the genome SAMHD1, a target gene knockout mutant is formed, and the aim of stably knocking out the SAMHD1 genes from the genome is achieved. |
| priorityDate | 2014-11-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 30.