http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104450762-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P17-00 |
| filingDate | 2013-09-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-03-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2018-03-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104450762-B |
| titleOfInvention | The biological synthesis method of alpha lipoic acid, engineered strain and preparation method thereof |
| abstract | The invention discloses a kind of biological synthesis method of alpha lipoic acid, engineered strain and preparation method thereof, the biological synthesis method is：Clone E. coli lipD genes, lplA genes, metK genes and lipA lipoic acid synthase genes；Build lipoic acid domain protein Overexpression vector；LplA series connection is cloned on the carrier of high efficient expression domain protein, ensures that domain protein is completely octanylated；Build lipA or with metK contact expression vector；Above-mentioned 4 kinds of carriers are transformed into suitable coli strain, adjusts and compares medium component and condition of culture, promote octanylated domain protein to the octanylated transformation of sulphur.Alpha lipoic acid preparation method of the present invention has to be combined using low value chemical raw material with pure biological production, and noxious material is few in production process, and environmental pollution is small, and it is synthesized, and lipoic acid is safe, and yield is high；Obtained alpha lipoic acid activity is high. |
| priorityDate | 2013-09-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 1364.