http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104447977-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-51 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-51 |
| filingDate | 2013-09-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2017-09-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2017-09-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104447977-B |
| titleOfInvention | A kind of production method for purifying of rhBMP2 |
| abstract | The invention discloses a kind of production method for purifying of rhBMP2.This method comprises the following steps：1）The inclusion body containing the albumen of rhBMP 2 is cracked, inclusion body lysate is obtained；2）Affinity chromatography and cation-exchange chromatography are carried out successively to the inclusion body lysate, isolates and purifies and obtains being denatured rhBMP2 solution；3）Renaturation is carried out to denaturation rhBMP 2 albumen, the albumen crude products of renaturation rhBMP 2 are obtained；4）Affinity chromatography is carried out successively to the albumen crude products of renaturation rhBMP 2 and gel permeation chromatography is isolated and purified, the albumen sterlings of renaturation rhBMP 2 are obtained.The albumen of rhBMP 2 is purified using method provided by the present invention, the product purity of preparation is up to more than 95%, and protein yield is 9.4%, endotoxin and DNA residuals meet bio-pharmaceutical quality requirement, and the inventive method is produced suitable for industrial scale；The development ＆ production also for similar recombinant protein medicine provides reference simultaneously. |
| priorityDate | 2013-09-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 54.