http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104436182-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-106 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P31-04 |
| filingDate | 2014-12-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2017-02-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2017-02-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104436182-B |
| titleOfInvention | Preparation method of ultrasonically broken bacterial component subunit vaccine of aeromonas hydrophila |
| abstract | The invention discloses a preparation method of an ultrasonically broken bacterial component subunit vaccine of aeromonas hydrophila. The preparation method disclosed by the invention comprises the following steps of: ultrasonically breaking bacterium solution in an ice bath by a phi 6 amplitude-change pole of an ultrasonic crusher under the conditions that the concentration of the selected bacterium solution is 2.4*10<9> CFU/mL, wherein the crushing condition is that the ultrasonic output power is 400W, the single radiation time is 12s and the ultrasonic interval time is 16s; the total radiation time is 16min; and then, the soaked vaccine of which the crushing ratio is up to 94% and simultaneously the antigen valence is kept at 1/320 is obtained. By means of the preparation method disclosed by the invention, a lot of subunit bacteria fragments can be obtained; the vaccine absorption ratio of fishes when the fishes are soaked in the vaccine to be vaccinated is increased; the bacterial antigenic activity is kept to the most extent; compared with the traditional all-bacteria soaked vaccine, the vaccine has the advantage that the relative immune protective rate is up to 38.14%; and a good solution is provided for preparing the bacterial component subunit soaked vaccine of aeromonas hydrophila and preventing ruinous bacterial septicaemia in fishery production. |
| priorityDate | 2014-12-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 22.