http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104404077-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-865 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-81 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-19 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 |
| filingDate | 2014-11-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-11-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2018-11-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104404077-B |
| titleOfInvention | A method of multiple foreign genes are cloned into microbial genome simultaneously |
| abstract | It is multiple foreign genes first to be imported into multiple two-way expression vectors, then multiple two-way expression vectors are imported into host microorganism simultaneously the invention discloses a kind of method that multiple foreign genes are cloned into microbial genome simultaneously.Each two-way expression vector includes two-way terminator and bidirectional promoterï¼›In addition to first two-way expression vector and the last one two-way expression vector, with the genome of host microorganism homologous recombination can occur for 5 ' the ends homology arm having the same of the 3 ' ends and next two-way terminator of two-way expression vector of other each two-way two-way terminators of expression vector, 5 ' ends of first two-way two-way terminator of expression vector and 3 ' ends of the last one two-way two-way terminator of expression vector.In this way, establish it is a kind of whole gene is expressed into process, including the new technology that the processes such as design of expression, clone's design, transform host cell organically combine. |
| priorityDate | 2014-11-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 22.