http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104388598-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6851 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-70 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-93 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2014-12-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2016-08-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2016-08-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104388598-B |
| titleOfInvention | A kind of HBV cccDNA digital pcr immue quantitative detection reagent box and application thereof |
| abstract | The invention belongs to the outer nucleic acid detection technique field of virion, be specially a kind of HBV cccDNA digital pcr immue quantitative detection reagent box and application thereof.First the present invention designs HBV cccDNA digital pcr detection by quantitative probe compositions, and test kit includes the container containing detection probe compositions and containing liver puncture tissue DNA extract purified reagent container.The step that this test kit uses includes: extracts DNA in liver puncture tissue, and uses PSAD enzyme to digest HBV rcDNA；With digital pcr detection chip as reaction vessel, composite fluorescence multiple PCR primer is used in digital pcr chip detection micropore, target DNA to be expanded；According to the fluorescence signal in each micropore of data PCR detection chip, obtain the quantitative result of HBV cccDNA in each cell.High specificity of the present invention, highly sensitive, easy, quick, it is not necessary to HBV cccDNA and HBV rcDNA is carried out extraction, overcome the defect in tradition HBV cccDNA detection, be suitable to large-scale promotion application. |
| priorityDate | 2014-12-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 25.