http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104388392-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-91 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-577 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-44 |
| filingDate | 2014-10-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2017-07-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2017-07-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104388392-B |
| titleOfInvention | A kind of enrofloxacin monoclonal antibody and its preparation method and application |
| abstract | The invention provides a kind of enrofloxacin monoclonal antibody and its preparation method and application.Illustrate, the present invention makees immunogene and envelope antigen simultaneously with Enrofloxacin and its with the conjugate of Cationic bovine serum albumin, indirect chessboard ELISA method is set up to demarcate suitable envelope antigen concentration and antiserum working concentration, affinity of the primary antibody to be measured to Enrofloxacin standard specimen is analyzed through indirect competitive ELISA again, is more than 1 with antiserum working concentration:50000 and 503nhibiting concentration IC 50 No more than 5ng/ml selects candidate mouse as index, obtains hybridoma cell strain 14H4A1 and 14F8B1 by screening using cell-fusion techniques and through strict, two plants of cells induce ascites, and then obtain target monoclonal antibody.The enrofloxacin monoclonal antibody with high titre and high-affinity that the present invention is provided, realizes the actually detected of Enrofloxacin denier residual, to realize that efficiently quick detection provides technical support and material base at scene. |
| priorityDate | 2014-10-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 36.