http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104388386-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0783 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P35-00 |
| filingDate | 2014-09-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2017-02-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2017-02-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104388386-B |
| titleOfInvention | In-vitro activation amplification method for V delta 1 gamma delta T cell coming from peripheral blood |
| abstract | The invention discloses an in-vitro activation amplification method for V delta 1 gamma delta T cell coming from peripheral blood. The method comprises: getting peripheral whole blood, employing a density gradient centrifugation and immunomagnetic bead sorting method to collecting total gamma delta T cell; resuspending the total gamma delta T cell by using an improved 1640 medium as a culture solution, performing cell culture under the conditions of 5% CO2 and 37 DEG C, and replacing a fresh culture solution every 2-3 days, so as to obtain in-vitro activated amplified gamma delta T cell mainly containing V delta 1 gamma delta T subset. The raw materials are convenient to obtain, the method does not cause an ethical issue and has extremely strong usage value. The obtained cell has relatively high cytotoxic activity of killing solid tumor cells, tumor stem cells and blood system tumor cells. The method for obtaining the cell is simple, efficient and low in cost. |
| priorityDate | 2014-09-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 93.