http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104374601-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N1-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N1-06 |
| filingDate | 2014-12-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-02-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2018-02-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104374601-B |
| titleOfInvention | A kind of resin slicer preparation method of kernel maturity seed |
| abstract | The invention discloses a kind of resin slicer preparation method of kernel maturity seed, including draw materials, fix, rinsing, being dehydrated, resin penetration, embedding, polymerize, repair the steps such as block, semithin section and dyeing, being characterized in：1. employ the secondary technique for fixing of glutaraldehyde fixer of 4% paraformaldehyde 2.5%；2. the London white glue that viscosity is very low, penetrating power is very strong is used（LR W hite）Resin is as embedding medium；3. establish a kind of dry incision technology of mature seed；4. establish a kind of 45 DEG C of 60 DEG C of two levels exhibition piece method.Instant invention overcomes conventional method make mature seed section severe curl, piece can not be opened up, be easily broken and iodine staining can not be carried out the shortcomings that, it is complete, clear that tissue, eucaryotic cell structure can be obtained, the obvious kernel maturity seed resin slicer of contrast, so as to provide accurate technical support to study the 26S Proteasome Structure and Function of mature seed. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108332989-A |
| priorityDate | 2014-12-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 29.