http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104372086-B

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classificationCPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6858
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filingDate 2014-11-06-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2016-05-18-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2016-05-18-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-104372086-B
titleOfInvention A kind of primer and method of fast detecting fugu obscurus juvenile fish gender differences single base mutation
abstract The invention discloses a kind of primer and method of fast detecting fugu obscurus juvenile fish gender differences single base mutation, the present invention is directed on the individual sex differential gene of fugu obscurus male and female and have single nucleotide mutation site, designed the pair of outside PCR primer of the gender differences gene order that increases respectively and contained the inner side PCR primer for the base mismatch in mutational site. Utilize described primer, fugu obscurus different sexes DNA sample can obtain different amplifications, thereby judges fugu obscurus gender differences. The present invention is with low cost, simple to operate, quick, accurate and be suitable for applying, can on the basis of extracting fugu obscurus genomic DNA in 1 age, quick and precisely identify there is important using value to accelerating fugu obscurus unisexuality seed selection process and biology field PCR detection method research fast to the sex of fugu obscurus juvenile fish.
priorityDate 2014-11-06-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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