http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104365474-A
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_aebe1d15574b4d4f5d787625dbbb368a |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01G1-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H1-06 |
filingDate | 2014-11-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e017d403cb0a060494bad3ecfe6a50ff http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bca93ee661509072221f777c1ca23da8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e5c33391fcebd60781faa04dbeaea68f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_130e4b4a2b806ac946e38176c6e0da32 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8355ef06919b8c578c461ac830696c5b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_70042fc1411bfbeffb2fb66f0cdc2ede http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3f0bccbf3cb7eec2275030a8bb5b2c86 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_85942e5210b4da90014589e0a2765d14 |
publicationDate | 2015-02-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-104365474-A |
titleOfInvention | Method applied to chemical mutagenesis of adventitious buds of bananas |
abstract | The invention discloses a method applied to chemical mutagenesis of adventitious buds of bananas. The method comprises the following steps: firstly, preparing a phosphate buffer solution with the pH value being 3.0, preparing a mutagenic agent sodium azide solution by the phosphate buffer solution with the pH value being 3.0, selecting the third and fourth generations of clustered adventitious buds of the adventitious buds of the bananas, cutting the clustered adventitious buds into single adventitious buds, putting the adventitious buds into the sodium azide solution for immersing and carrying out mutagenesis, transferring the adventitious buds subjected to mutagenesis to a proliferation medium, putting the proliferation medium in an axenic cultivation chamber for carrying out recovery culture for about 20 days; transferring the survival adventitious buds subjected to the recovery culture into a rooting medium, strengthening seedlings and rooting, and carrying out rooting culture for about 25 days; transferring rooting seedlings into a seedling culturing greenhouse and hardening the seedlings for 5 days, then strengthening seedlings and rooting in a temporary planting sand tank for 20 days, then planting the seedlings in a seedling culturing tray; and screening variant strains when ten leaves of the seedlings in test tubes grow. The method has the beneficial effects that the banana breeding has the characteristics of being high in mutagenesis frequency, wide in range, quick to separate and easy to stabilize. |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2022110864-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112293248-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112753572-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112369323-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112293248-A |
priorityDate | 2014-11-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 41.