http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104365474-A

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publicationDate 2015-02-25-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-104365474-A
titleOfInvention Method applied to chemical mutagenesis of adventitious buds of bananas
abstract The invention discloses a method applied to chemical mutagenesis of adventitious buds of bananas. The method comprises the following steps: firstly, preparing a phosphate buffer solution with the pH value being 3.0, preparing a mutagenic agent sodium azide solution by the phosphate buffer solution with the pH value being 3.0, selecting the third and fourth generations of clustered adventitious buds of the adventitious buds of the bananas, cutting the clustered adventitious buds into single adventitious buds, putting the adventitious buds into the sodium azide solution for immersing and carrying out mutagenesis, transferring the adventitious buds subjected to mutagenesis to a proliferation medium, putting the proliferation medium in an axenic cultivation chamber for carrying out recovery culture for about 20 days; transferring the survival adventitious buds subjected to the recovery culture into a rooting medium, strengthening seedlings and rooting, and carrying out rooting culture for about 25 days; transferring rooting seedlings into a seedling culturing greenhouse and hardening the seedlings for 5 days, then strengthening seedlings and rooting in a temporary planting sand tank for 20 days, then planting the seedlings in a seedling culturing tray; and screening variant strains when ten leaves of the seedlings in test tubes grow. The method has the beneficial effects that the banana breeding has the characteristics of being high in mutagenesis frequency, wide in range, quick to separate and easy to stabilize.
isCitedBy http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2022110864-A1
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priorityDate 2014-11-18-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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